HK2 and ischemia: qPCR analysis of hind limb tissues before ischemia in the two groups of mice showed that KD significantly decreased glycolysis by decreasing GLUT4, GLUT1, and HK2 gene expression and increasing PDK1 gene expression (Fig. 6A), while it increased fatty acid utilization by increasing CD36 and CPT1 gene expression compared with ND mice (Fig. 6B).