PECAM1 and ischemia: Further WB tests showed that both CD31 and VEGFA protein expression levels significantly decreased in the hind limb tissue of KD mice following ischemic surgery compared with mice in the ND group, but no significant difference was found in limb tissue between the KD and ND groups before ischemic surgery, indicating that KD reduced revascularization in hind limb tissue after ischemia (Fig. 2F).