ROC analysis demonstrated that autoantibody to SPP1 was obvious to distinguish patients with ESCC from NC, which had an AUC of 0.653 (95%CI: 0.556–0.750) with the sensitivity (Se) of 45.16% and specificity (Sp) of 83.87% (Fig. 4B, Table 4). This evidence concerns the gene SPP1 and esophageal squamous cell carcinoma.