To test whether suppression of PD-L1 can enhance the killing effect of NK cells and further inhibit PCa tumor growth under BAT in vivo, we generated EnzR1-C4-2 cells with stable expression of sh-PD-L1 or vector control (pLKO) cells and subcutaneously implanted these cells into nude mice for 4 treatment groups for each cell line as follows, 1: pLKO+EtOH; 2: pLKO+Testosterone; 3: sh-PD-L1 + EtOH; and 4: sh-PD-L1 + Testosterone (Fig. 7A). The gene discussed is CD274; the disease is neoplasm.