To further confirm the enhanced inflammatory process during pneumococcal meningitis with an impaired glymphatic system's functionality, we also measured by Western blot analysis the levels of interferon gamma (IFN-γ), a cytokine with a critical role in potentiating the proinflammatory signaling by priming macrophages and microglia during infections (16, 17). Here, IFNG is linked to pneumococcal meningitis.