Additionally, H&E staining also revealed adipose tissue formation in DMD-COs on day 28 (Figure 5D), confirmed by the detection of lipid droplets via BODIPY staining and immunolabelled PDGFRα+ cells (an adipocyte marker) in DMD-COs on day 28 and 56 (Figure 5E). This evidence concerns the gene DMD and Duchenne muscular dystrophy.