HSPA5 and obesity due to melanocortin 4 receptor deficiency: ASCs were collected 48 h after coculture, and results showed that the mRNA expression and protein levels of senescent (P16 and P21) and SASP (IL6 and Ccl2) markers and ER stress-related genes (sXbp1 and Grp78) (Figures 3(g) and 3(j)) were significantly higher in the ASCs cocultured with adipocytes from the hypertrophic obesity mice than in the ASCs cocultured with adipocytes from the control mice.