We next validated the relationship between H3K36 methylation regulatory genes (SETD2, SETD3, NSD1, PAXIP1, BRD4, IWS1, SETMAR, SMYD2, ASH1L) and SF2 in the dataset GSE5949 (pan-cancer cell lines, n = 59) (Reinhold et al., 2010). The gene discussed is IWS1; the disease is cancer.