By comparison, the minimal expression of CHD3 and DYRK2 were observed in healthy living donor samples, and early stage DKD samples encountered the weakest expression of ATAD2, NFKB1, and YAP1. These expression patterns of hub genes proposed CHD3 and DYRK2 as the putative early diagnostic biomarkers for DKD, while ATAD2, NFKB1, and YAP1 as putative biomarkers for monitoring DKD progression. The gene discussed is CHD3; the disease is diabetic kidney disease.