CD8A and osteosarcoma: We evaluated distribution of CD8A was in the endoplasmic reticulum (ER) and microtubules (MT) in the alveolar rhabdomyosarcoma RH-30, human neuroblastoma SH-SY5Y, and human osteosarcoma U-2 OS cell lines, and we discovered that CD8A protein overlapped with ER and MT but was not expressed in the nucleus of 3 cell lines, indicating its subcellular location in the plasma membrane (Figure 2D).