Then, it was predicted and verified that one of the target genes of miR-1-3p is SLC8A1/NCX1, which is related to cardiomyocyte apoptosis, indicating that circulating miRNAs involved in the regulation of VSD may be derived from the myocardium, providing a theoretical basis for the use of circulating miRNAs to assist in the diagnosis of VSD. Here, SLC8A1 is linked to ventricular septal defect.