It was observed that all 16 genes (TP53, TTN, LRP1B, ZFHX4, MUC16, OBSCN, CSMD3, FLG, CSMD1, SYNE1, SPTA1, USH2A, KMT2C, PCLO, HMCN1, and FAT3) were significantly associated with higher TMB in patients with HCC (Figure 2). The gene discussed is CSMD1; the disease is hepatocellular carcinoma.