SPTA1 and hepatocellular carcinoma: It was observed that all 16 genes (TP53, TTN, LRP1B, ZFHX4, MUC16, OBSCN, CSMD3, FLG, CSMD1, SYNE1, SPTA1, USH2A, KMT2C, PCLO, HMCN1, and FAT3) were significantly associated with higher TMB in patients with HCC (Figure 2).