It was observed that all 16 genes (TP53, TTN, LRP1B, ZFHX4, MUC16, OBSCN, CSMD3, FLG, CSMD1, SYNE1, SPTA1, USH2A, KMT2C, PCLO, HMCN1, and FAT3) were significantly associated with higher TMB in patients with HCC (Figure 2). This evidence concerns the gene HMCN1 and hepatocellular carcinoma.