We then performed the multivariate survival analysis of all variables (stage, age, and MUC16 mutation status) with P < 0.05 in univariate analysis by Cox proportional hazards analysis, and the result showed that age (HR = 1.65, 95%CI = 1.18–2.31; P=0.003), stage (HR = 2.18, 95%CI = 1.54–3.08; P < 0.001), and MUC16 mutation (HR = 0.64, 95%CI = 0.44–0.93; P=0.018) were independent prognostic factors in individuals with HCC (Table 3). Here, MUC16 is linked to hepatocellular carcinoma.