In this work, with the use of quantitative mass spectrometric proteomics analysis, we revealed for the first time that the classic traditional Chinese medicine, Descurainia sophia seeds (DS) relieved isoproterenol-induced cardiomyopathy (ISO-iCM) in rats by reversing the most of differentially expressed proteins (DEPs), including molecular motor MYL4 and the well-known biomarker NPPA of cardiomyopathy. The gene discussed is MYL4; the disease is cardiomyopathy.