Since the most of the 25 DEPs, including eight extracellular matrix proteins and six myosin family proteins, and five actin-binding proteins, are involved in the cytoskeleton and myofibril structure and function, the disorders in cardiac muscle contraction, in particular structure and function of sarcomeric myosin motor, is most likely the main pathogenic elements of ISO-iCM in rats, similar to DCM and HCM in human. Here, MYH16 is linked to familial dilated cardiomyopathy.