To evaluate cell death with an orthogonal method, we intravenously injected a near-infrared fluorescent Annexin-V molecular imaging probe, which binds to phosphatidyl serine on the surface of apoptotic cells and in the interior of necrotic cells33, 4 hours after MI and harvested the hearts 60 minutes later. The gene discussed is ANXA5; the disease is myocardial infarction.