Several high-frequency gene mutations (FRG1B, SPOP, and TTN) were identified in the TCGA PCa cohort, in which FRGPI-high patients showed significantly higher mutation counts than FRGPI-low patients.FRG1B (FSHD region gene 1 family, member B) displayed the highest mutation frequency in line with previous reports that well-distributed missense mutations of FRG1B are frequently detected in PCa and glioma. This evidence concerns the gene SPOP and central nervous system cancer.