Meanwhile, we confirmed the hub genes of livers from NASH and corresponding control mice by qRT-PCR and found that COL1A1, IGFBP7, COL1A2, Cercam, COL5A1, Agpat4, STING1, RHBDF1, SPON1, COL3A1,FKBP10, Mxra8 andTIMP1were significantly increased in HFHC fed mice, while PTGDS was reduced in the NASH group in comparison with the control group (Figures 5D, E). The gene discussed is RHBDF1; the disease is metabolic dysfunction-associated steatohepatitis.