Meanwhile, we confirmed the hub genes of livers from NASH and corresponding control mice by qRT-PCR and found that COL1A1, IGFBP7, COL1A2, Cercam, COL5A1, Agpat4, STING1, RHBDF1, SPON1, COL3A1,FKBP10, Mxra8 andTIMP1were significantly increased in HFHC fed mice, while PTGDS was reduced in the NASH group in comparison with the control group (Figures 5D, E). This evidence concerns the gene COL5A1 and metabolic dysfunction-associated steatohepatitis.