In addition, the value of these genes in diagnosing NAFLD were also speculated and we found that RHBDF1, COL3A1, QSOX1 and STING1 showed a significant diagnosis power with AUC more than 0.8, while COL5A1, IGFBP7, COL4A2, COL1A1, FKBP10 and COL1A2 also came out well with AUC more than 0.7 (Figure 4F). The gene discussed is RHBDF1; the disease is metabolic dysfunction-associated steatotic liver disease.