It is possible that the low pH value in malignant tumor cells destabilizes the binding of iron to transferrin and allows iron to escape from transferrin Fe3+ to Fe2+ after more Fe3O4 enters the tumor cells (21).The designed strategy could result in more ferroptosis in the cancer cells by synergistic effects of the cytotoxicity of ART and increased intracellular levels of Fe2+ ions (6; 22). This evidence concerns the gene TF and cancer.