In addition, we analyzed available ChIP-seq data (GSE147074) with or without ASH1L knockout in anaplastic thyroid carcinoma BHT-101 cells and found that ASH1L knockout significantly reduced the H3K36me2 enrichment at some oncogene loci including APLP2, SERPINE1, NEK7, LAMB3, and ITGA6 (Supplemental Figure S3C) and consequently modulated the expressions of these genes (37). Here, APLP2 is linked to thyroid gland undifferentiated (anaplastic) carcinoma.