To explore the biological function of ASH1L in cancers, we evaluated the ASH1L protein levels in different cancer cell lines, such as androgen-sensitive PCa LNCaP, androgen receptor (AR)–negative CRPC (PC3, DU145), human cervical carcinoma cell line C33A, human bone osteosarcoma epithelial cell line U2OS, human pancreatic carcinoma cell line MIA-PaCa2, human gastric cancer cell line AGS, and human embryonic kidney cell line 293T. Here, ASH1L is linked to osteosarcoma.