To understand the differences in immune cell subset expression in the glioma immune microenvironment, we calculated and compared the expression of different infiltrating immune cells, as shown in Figure 6A; Activated dendritic cells (aDCs), B cells, CD8+ T cells, DCs, Macrophages, NK cells, Plasmacytoid DCs (pDCs), T helper cells, Tfh, Th2 cells, TILs, and Tregs were significantly upregulated in the high RS group. The gene discussed is CD8A; the disease is glioma.