AR and Familial prostate cancer: In order to explore the role of AR in NEHIGH prostate cancer cells, we extracted cells in NEHIGH clusters (clusters 0, 3, 6, 9, 15, 20, and 22) and divided them into the ARHIGH/NEHIGH and ARLOW/NEHIGH groups according to the expression level of AR. Within the cells from NEHIGH clusters, the median expression level of AR was first determined, and cells with AR expression higher than the median level were attributed to the ARHIGH/NEHIGH group, while the rest were attributed to the ARLOW/NEHIGH group.