AFP and neoplasm: Using negative enrichment with CD45- and iFISH®, CTCs were detected in 26 (52%) patients and CTCs count positively correlated with several other prognostic factors like tumor size (χ2 = 5.77, p = 0.016), AFP level (χ2 = 5.45, p = 0.02) and tumor grade (χ2 = 6.48, p = 0.039).