Then, further subgroup analysis exhibited that in >60 years old [0.228 (0.487–2.486) vs 1.048 (0.143–14.076), P = 0.016], male [0.226 (0.027–3.267) vs 0.821 (0.097–12.904), P = 0.013], smoker [0.235 (0.025–2.695) vs 0.737(0.061–14.324), P = 0.007], LUSC [0.126 (0.017–2.486) vs 0.556 (0.059–11.459), P = 0.002], and stage II–IV subgroup [0.205 (0.019–3.697) vs 0.905 (0.081–13.452), P = 0.012], BZRAP1-AS1 was lower in tumor tissues than adjacent normal tissues. This evidence concerns the gene TSPOAP1 and neoplasm.