The virulence factors spsE, hlgA, siet, and PVL were detected in more (P < 0.05) of the strains isolated from the dogs with keratitis (93.3, 86.7, 100, and 93.3%, respectively) compared with the strains isolated from the healthy dogs (50, 64.3, 92.3, and 85.7%, respectively). This evidence concerns the gene GLS2 and keratitis.