In order to examine the therapeutic efficacy of MCC950 in vivo and whether it may possess a synergistic effect if combined with immune checkpoint immunotherapy, WT animals were inoculated with B16.F10 melanoma cells and treated with MCC950 alone or in combination with anti-PD-1 and anti-CTLA-4 monoclonal antibodies (Figure 8A). This evidence concerns the gene CTLA4 and melanoma.