Collectively, compared to the analog vaccination settings, tumor-reactive CD8 T cells recruited after vaccination with the native EAA peptide are of reduced frequency (13), yet they show a higher T cell clonotype diversity with subtle CDR3β structural differences (72), have TCRs of higher structural avidities linked to CD8-independancy and enhanced function (31), and are in average more cross-responsive to epitope-focused peptide variants (1 or 2 amino-acid substitutions from the wild-type sequence) having widespread Ki affinity (this study). The gene discussed is CD8A; the disease is neoplasm.