Discovery of attenuated B-cell antigen presentation, distinct regulatory T-cell clusters with different origins and novel polyfunctional tumor related SPP1+ tumor-associated macrophages (TAMs)Identification of increased XCL1+ in T-cell clusters (CD8+ cytotoxic T lymphocytes and exhausted CD8+ T cells) linked with high status of tumor mutationsContribution of tumor mutational burden (TMB) in shaping CRC immune landscapeStudy of molecular mechanisms/epigenetic shaping of the tumor immune microenvironment via single-cell assay for transposase accessible chromatin using sequencing (scATAC-seq). Here, SPP1 is linked to colorectal carcinoma.