Our data showed that LD96.24 increased the ratio of Foxp3+ Tregs to IFN-γ+ Teffs and delayed the development of T1D, which indicated that the ratio of Foxp3+ Tregs T-cells to IFN-γ+ Teffs might be an indicator of the development of T1D. Here, IFNG is linked to type 1 diabetes mellitus.