INS and Insulin resistance: In the muscle of ob/ob mice, the expression of AKT2 was lower, and insulin resistance was observed in vitro.110 The PI3K inhibitor wortmannin fully inhibits insulin-stimulated glucose uptake in skeletal muscle.111 RalGAPα1 mainly exists in skeletal muscle, blunts insulin effects by preventing translocation of GLUT4, and can be inactivated by AKT.