In the muscle of ob/ob mice, the expression of AKT2 was lower, and insulin resistance was observed in vitro.110 The PI3K inhibitor wortmannin fully inhibits insulin-stimulated glucose uptake in skeletal muscle.111 RalGAPα1 mainly exists in skeletal muscle, blunts insulin effects by preventing translocation of GLUT4, and can be inactivated by AKT. Here, AKT1 is linked to Insulin resistance.