A CHMP4-bound Alix dimer, mediated by the V domain, has been implicated in HIV-1 budding and MVB sorting of the activated EGFR (3, 13), while the 852PSYP855 motif has been found necessary for Alix’ role in virus infection (36), and HIV budding at the plasma membrane. This evidence concerns the gene PDCD6IP and viral infectious disease.