As shown in Figure 8, cytotoxic T lymphocyte‐associated antigen 4 (CTLA‐4), programed death 1 (PD‐1), and PD‐L1 were positively associated with ANLN expression in multiple cancers, including BRCA, KICH, KIRC, LIHC, PRAD, and THCA. This evidence concerns the gene CD274 and cancer.