Our results demonstrated that ANLN expression was significantly associated with the infiltration levels of macrophages M1 in 18 cancer types (Figure 6, those with p < 0.00001 were shown), CD4 + T cells in 12 cancer types, CD8+ T cells in 9 cancer types, T cells regulatory (Tregs) in 13 cancer types, B cells in 8 cancer types, M2 in 7 cancer types, NK cells in 9 cancer types, and neutrophils in 7 cancer types (those with p < 0.00001 were shown in Figure S2). This evidence concerns the gene CD8A and cancer.