Immunohistochemical staining of cardiac tissues from CH group rats revealed significant downregulation of LC-3 protein expression (p < 0.001) (Fig. 3I, ii) and significant upregulation of P62 protein expression (p < 0.001) compared to the Control group (Fig. 3I i, J i, respectively) as measured by % positive area. Here, SQSTM1 is linked to cyclic hematopoiesis.