To analyze whether aPC reverses tubular injury and senescence via DNMT1 in vivo, we reduced blood glucose levels in diabetic mice using SGLT2i starting at week 16 post induction of hyperglycemia for further six weeks (DM + SGLT2i), adding aPC in a subgroup of mice (DM + SGLT2i + aPC+PBS, Fig. 7a, b). This evidence concerns the gene DNMT1 and Hyperglycemia.