We performed single-cell immune repertoire sequencing on immunodominant NP396+CD8+ T cells of mice receiving only a neonatal i.c. infection (carrier-non-challenge (CNC), n = 1) and mice receiving both an i.c. infection and an i.v. infection with wtLCMV following 6 weeks and sacrificed after either 10 or 25 days (Carrier challenge [CC10 and CC25, respectively], n = 3 for each time point) (Fig. 3A). This evidence concerns the gene CD8A and infection.