Klhl3+/+ and Klhl3−/− mice fed the MCD diet for 6 weeks developed severe steatosis, lobular inflammation, and hepatocellular ballooning in the liver compared to controls fed a NC diet; however, the NAFLD activity was significantly decreased in the livers of Klhl3−/− mice compared to the Klhl3+/+ mice when both groups were fed the MCD diet (Fig. 3a–d). Here, KLHL3 is linked to steatosis.