Metastatic melanoma is characterized by high levels of matrix metalloproteinase (MMP)‐2 and ‐9 secretions that decompose the surrounding ECM, allowing melanoma cells to spread to other organs.[22] Therefore, to investigate the release level of MMPs by the printed spheroids, the amounts of MMP‐2 and MMP‐9 were analyzed through enzyme‐linked immunosorbent assay (ELISA). This evidence concerns the gene MMP2 and melanoma.