One is to inoculateAHNAK2-knockdown MDA-MB-231 cellsin situ into the mammary fat pads of nude mice, and the other is to injectAHNAK2-knockdown Hela cells subcutaneously into nude mice.AHNAK2 knockdown dramatically delayed tumor formation and suppressed tumor growth in the Hela tumor xenograft model, as shown by reduced tumor volume and tumor size (Figure 4E). This evidence concerns the gene AHNAK2 and neoplasm.