INS and type 2 diabetes mellitus: HFD mice successfully developed T2DM symptoms, as the mean FBG levels were at 8.01 mmol/ml (p = 0.01) with a significantly higher serum insulin level (p = 0.000037) and a higher area under the curve (AUC) of GTT (p = 0.000001) and ITT (p = 0.004) than the ND group (Figures 1F–I).