In our study, PSG was found to protect the intestinal barrier by increasing the expressions of tight junction protein ZO1 and occludin of IBD mice, reduce the level of LPS in liver tissues, and suppress the LPS/TLR4/MyD88/NF-κB signaling pathway, implying that PSG could protect the colon from damage, improve the intestinal permeability, and also found to prevent IBD-associated liver damage through regulating the gut-liver axis. Here, TJP1 is linked to inflammatory bowel disease.