In ePL medium, the VEGF-A release was further increased in co-culture, so that the highest VEGF-A concentrations were measured in the equine AD-MSC and endothelial cell co-culture with ePL medium, suggesting a synergism of ePL and AD-MSC in providing high concentrations of VEGF-A. The gene discussed is VEGFA; the disease is Alzheimer disease.