Clinical biomarkers such as gender, age, BMI, the distance from the anal verge to the lower edge of the tumor, T stage, N stage, and mrCRM, and pretreatment blood biomarkers such as serum albumin, serum prealbumin, hemoglobin, PLR, SII, and PNI were not associated with pCR to NCRT (all P > 0.05). The gene discussed is ALB; the disease is neoplasm.