There was no difference in age, height, weight, BMI, abstinence days, semen volume, total sperm count (TSC), hormone (including follicle stimulating hormone, luteinizing hormone, prolactin, estradiol, and testosterone), and seminal plasma biochemical components (including neutral α-glucosidase, citric acid, and zinc) of the patients with different varicocele locations before treatment (p > 0.05) (Table 5). This evidence concerns the gene PRL and varicocele.