The results showed that there was no difference in the indicators of age, weight, height, BMI, abstinence days (AD), mean varicocele width (MVW), semen volume (SV), pH value, total sperm count (TSC), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone, neutral α-glucosidase, citric acid, and zinc in patients with different varicocele locations before and after the treatment (p > 0.05) (Table 4). The gene discussed is PLOD1; the disease is varicocele.