Similar to CRP, the mRNA level of TNF-α in rRGNNV-B2-M1 and rRGNNV-B2-M2 infected zebrafish was also significantly lower than that in wtRGNNV and rRGNNV-infected zebrafish with higher virus titer (1.45 × 106 TCID50/mL and 1.45 × 105 TCID50/mL) infection. This evidence concerns the gene TNF and infection.