To further examine which stage of the WSSV life cycle was regulated by the deacetylase activity of CqSIRT1, the representatives of different stages of WSSV infection, including the immediately early gene ie1, the later viral envelope gene vp28 and the later viral nucleocapsid gene vp664, in Hpt cells following WSSV infection at different time points were analyzed. The gene discussed is MRS2; the disease is infection.