After the selection of the most significant genes using GSEA and the DisGeNET database, we decided to test the expression levels of HRAS, CTTN, ACTN1, EPB41L2, and YKT6, which are involved in carcinoma (HRAS), tumor invasion (CTTN, YKT6), and cirrhosis (EPB41L2, ACTN1) and cross-validate them with the mass spectrometry results. The gene discussed is ACTN1; the disease is Cirrhosis.