Hence, different cut-off values of AFP for detecting HCC should be referred in populations with a different status of HBV suppression and liver inflammation, and receiving antiviral therapy and normal of AST would be the two important and referable indicators to conduct a judgement and selection on the specific cut-off values, which appears to be particularly important, as more and more patients with HBV infection receive highly potent NAs antiviral therapy. The gene discussed is AFP; the disease is hepatocellular carcinoma.