TNF and infection: To further investigate the type of response that was induced by immunization with rSs14-3-3 and rSsMA, followed by the infection, the levels of expression of selected cytokines (IFN-γ, TNF-α, IL-12, IL-4, IL-5, IL-13, IL-17, IL-10, TGF-β, IL-23) were measured in the spleen using qPCR.