In vivo biodistribution of [18F]AlF-NOTA-OncoFAP (500 nmol/kg, ~4 MBq/Kg, RCP 89%) in athymic Balb/c AnNRj-Foxn1 mice bearing subcutaneous HT-1080.hFAP fibrosarcoma showed selective accumulation in FAP-positive tumors (6.6% ID/g, 1 h post intravenous injection), with excellent selectivity against healthy organs. This evidence concerns the gene FAP and fibrosarcoma.