AKT1 and infection: Upon infection with non-neuropathogenic Jan-E and Rac-H EHV-1, the highest increase in ROS occurred at 2 h p.i., followed by a decrease observed at 24 h p.i. Our team previously showed that the Jan-E EHV-1 and Rac-H EHV-1 induced apoptosis only in a small population of cells [14].