AMPK and SIRT1 play important roles in a variety of physiological processes, including regulating energy expenditure [22], attenuating oxidative stress-induced apoptosis [57], regulating hyperglycemia-induced cell death [58], attenuating ethanol-induced liver injury [59], relieving non-alcoholic fatty liver disease [60] and activating lysosomal function in the brain [61]. Here, SIRT1 is linked to metabolic dysfunction-associated steatotic liver disease.