INT-777, as a special TGR5 agonist, reduced the severity of AP in mice, which was manifested as decreased pancreatic tissue damage as well as a decrease in the expression of serum enzymes (amylase and lipase), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and necrosis-related proteins (RIP3 and p-MLKL) caused by inhibiting the reactive oxygen species/nucleotide-binding oligomerization domain (NOD)-like receptor containing the pyrin domain (3ROS/NLRP3) inflammasome pathway [19]. Here, IL1B is linked to alkaline phosphatase measurement.