To evaluate the effect of the observed (likely) pathogenic mutations in CTNNB1 and APC in the two CCA cell lines CC-LP-1 and KKU-M055, Nanostring® gene expression analyses using the PanCancer Pathway Panel were conducted in comparison with the two WNT pathway WT cell lines HuCC-A1 and TKKK (Figure 4b,c). The gene discussed is CTNNB1; the disease is cholangiocarcinoma.